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Details of the history of inner Eurasia revealed by new study


An international team of researchers has combined archaeological, historical and linguistic data with genetic information from over 700 newly analyzed individuals to construct a more detailed picture of the history of inner Eurasia than ever before available. In a study published in Nature Ecology & Evolution, they found that the indigenous populations of inner Eurasia are very diverse in their genes, culture and languages, but divide into three groups that stretch across the area in east-west geographic bands.

Details of the history of inner Eurasia revealed by new study
Children from one of the Tajikistan communities included in the study
[Credit: Elena Balanovska]
Inner Eurasia, including areas of modern-day Armenia, Georgia, Kazakhstan, Moldova, Mongolia, Russia, Tajikistan, Ukraine and Uzbekistan, was once the cross-roads connecting Asia and Europe, and a major intersection for the exchange of culture, trade goods and genes in prehistory and historical periods, including the era of the famous Silk Road.

This vast area can also be divided into several distinct ecological regions that stretch in largely east-west bands across Inner Eurasia, consisting of the deserts at the southern edge of the region, the steppe in the central part, taiga forests further north, and tundra towards the Arctic region. The subsistence strategies used by indigenous groups in these regions largely correlate with the ecological zones, for example reindeer herding and hunting in the tundra region and nomadic pastoralism on the steppe.


Despite the long and important history of inner Eurasia, details about past migrations and interactions between groups are not always clear, especially in prehistory. "Inner Eurasia is a perfect place to investigate the relationship between environmental conditions and the pattern of human migration and mixture, as well as changes driven by cultural innovations such as the introduction of dairy pastoralism into the steppe," states Choongwon Jeong of the Max Planck Institute for the Science of Human History, co-first and senior author of the paper.

In order to clarify our understanding of some of the nuances of the history of the region, an international team of researchers undertook an ambitious project to use modern and ancient DNA from a broad geographic range and time period, in concert with archaeological, linguistic and historical information, to clarify the relationships between the different populations. "A few ethnic groups were studied previously," comments Oleg Balanovsky from the Vavilov Institute of General Genetics in Moscow, also co-first author, "but we conducted more than a hundred field trips to study this vast region systematically, and reached communities speaking almost all of the Inner Eurasian languages".

Three distinct east-west groupings

For this study, the researchers analyzed DNA from 763 individuals from across the region as well as reanalyzed the genome-wide data from two ancient individuals from the Botai culture, and compared those results with previously published data from modern and ancient individuals. They found three distinct genetic groupings, which geographically are arranged in east-west bands stretching across the region and correlating generally to ecological zones, where populations within each band share a distinct combination of ancestries in varying proportions.

Details of the history of inner Eurasia revealed by new study
Geographic locations of the Eneolithic Botai, groups including newly sampled individuals, and nearby groups
with published data. The map is overlayed with ecoregional information, divided into 14 biomes
downloaded from ecoregions2017.appspot.com/ (credited to Ecoregions 2017 © Resolve)
[Credit: Jeong & Balanovsky et. al. 2019]
The northernmost grouping, which they term "forest-tundra", includes Russians, all Uralic language-speakers, which includes Hungarian, Finnish and Estonian, and Yeniseian-language speakers, of which only one remains today and is spoken in central Siberia. The middle grouping, which they term "steppe-forest", includes Turkic- and Mongolic-speaking populations from the Volga and the region around the Altai and Sayan mountains, near to where Russia, China, Mongolia and Kazakhstan meet. The southernmost grouping, "southern-steppe", includes the rest of Turkic- and Mongolic-speaking populations living further south, such as Kazakhs, Kyrgyzs and Uzbeks, as well as Indo-European-speaking Tajiks.

Previously unknown genetic connections revealed

Because the study includes data from a broad time period, it is able to show shifts in ancestry in the past that reveal previously unknown interactions. For example, the researchers found that the southern-steppe populations had a larger genetic component from West and South Asia than the other two groupings. This component is also widespread in the ancient populations of the region since the second half of the first millennium BC, but not found in Central Kazakhstan in earlier periods. This hints at a population movement from the southern-steppe region to the steppe-forest region that was previously unknown.


"Inner Eurasia has functioned as a conduit for human migration and cultural transfer since the first appearance of modern humans in this region. As a result, we observe deep sharing of genes between Western and Eastern Eurasian populations in multiple layers," explains Jeong. "The opportunity to find direct evidence for the hidden old layers of admixture, which is often difficult to appreciate from present-day populations, is very exciting."

"We found not only corridors, but also barriers for migrations," adds Balanovsky. "Some of them separate the historical groups of populations, while others, like the distinct barrier following the Great Caucasus mountain ridge, were obviously shaped by the geographic landscape."

Two ancient individuals resequenced in this study originated from the Botai culture in Kazakhstan where the horse was initially domesticated. Analysis of the Y-chromosome (inherited along the paternal genealogical lines) revealed a genetic lineage which is typical in the Kazakh steppe up to the present day. But analysis of the autosomes, which both parents contribute to their children, show no trace of Botai ancestry left in present-day people, likely due to repeated migrations into the region both from the west and the east since the Bronze Age.

The researchers emphasize that their model of three groupings does not perfectly explain all known populations and that there are examples of both outliers and intermediate groups. "It is important to organize a future study for further sampling of sparsely populated regions between the clines, for example, Central Kazakhstan or East Siberia," states Johannes Krause, also of the Max Planck Institute for the Science of Human History, and senior author of the paper.

Source: Max Planck Institute for the Science of Human History [April 29, 2019]

Bactrian fortress reveals how ancient civilizations of Central Asia lived


Scientists from Russia and Uzbekistan found a unified fortification system that on the northern border of ancient Bactria. This country existed in the III century BC. The fortress found blocked the border and protected the oases of Bactria from the nomads raids. During the excavations, scientists revealed the fortress citadel, drew up a detailed architectural plan and collected rich archaeological material indicating the construction, life and death of the fortress as a result of the assault.

Bactrian fortress reveals how ancient civilizations of Central Asia lived
View of the Uzundara citadel from above [Credit: Nigora Dvurechenskaya]
In the IV century BC, significant part of Central Asia territory belonged to Bactria, which, as a separate satrapy, was part of the Achaemenid Empire. In 329 BC Bactria became part of the Alexander empire and after his death joined the kingdom of Seleucid: the largest Hellenistic state in the East, created by the commander Alexander Seleucius I Nicator and his son Antiochus I Soter. Gradually, the state became weakened by numerous military campaigns and the struggle for power. As a result, once flourishing Bactria ceased to exist in the II century BC when Iranian-speaking nomads from the northern territories, the Saki and Yuadzhi, broke into the country.

Recently, Russian scientists completed excavations in this area and determined the fortress construction time: about 95-90 years of the III century BC, the time of Antiochus I reign and the very beginning of the formation of the Seleucid state. The fortress was inhabited for about 150 years.


It consisted of a diamond-shaped main quadrangle, a triangular citadel (phylacterion), surrounded by powerful double walls with an internal gallery about nine meters wide, and extension walls, which were fortified with 13 rectangular bastions-towers, three of which were also outboards. Outside the fortress there was a marketplace where local residents brought goods needed by the garrison soldiers.

The archaeologists recorded the location of each item using a total station or GPS, and then made it into a single plan tied to the terrain. As a result, the scientists managed to establish where the marketplace was, ran the road to the entrance to the fortress, and determined the place of the assault: there were more than 200 shooting arrowheads, combat darts and troops. It is curious that the proposed battlefield is located to the east of the fortress, which suggests a possible environment or the breakthrough of the enemy through a system of border fortifications.


The warriors who defended Uzundar wore armor: in the inside-wall room of the south-western fortified wall, archaeologists discovered armor-clad plates and two right-handed iron heads from helmets. So far, scientists can not exactly determine what type of helmets these patches were -- a pseudoattical or Melos group, so it is still possible that these are the same helmets that Alexander wore during Antiochus I Soter period.

"This findings are sensational: direct analogies are known from the Takhti-Sanga temple, but there they were bronze, and we found iron fragments in Uzundar. To date, there are only a few specimens and sculptures with which to compare these cheeks and determine their type. We also found fastening details, which gives important information on manufacturing technology, according to tradition, but to answer these questions requires lengthy research," says Nigora Dvurechenskaya, researcher at the Department of Classical Archeology, Head of the Bactrian detachment of the Central Asian Archaeological Expedition.


In addition to weapons, archaeologists have collected a large number of ceramics, as well as a rich numismatic collection: today around the fortress found about 200 coins of very good preservation from the coins of Antiochus I and all the rulers of the Graeco-Bactrian kingdom from Diodot to Heliocles of very different denominations: from silver drachmas to copper mites. Such a variety proves that Bactria at the very beginning of Seleucid kingdom formation of the was part of developed monetary circulation system. Thus, the materials of Uzundara allow to study and reconstruct all spheres of life of the Seleucidian and Graeco-Bactrian fortresses.

Source: Akson Russian Science Communication Association [Febuary 01, 2019]

Mural found in Uzbekistan gives fresh glimpse of early Buddhism


A brightly colored mural unearthed in Uzbekistan likely dates from the second to third centuries and sheds intriguing light on the spread of Buddhist art along the Silk Road, researchers say.

Mural found in Uzbekistan gives fresh glimpse of early Buddhism
Part of the mural unearthed at the Kara Tepe archaeological site in Uzbekistan in 2016
[Credit: Rissho University Uzbekistan Academic Research Group]
It was discovered in 2016 during excavations at Kara Tepe, an archaeological site in suburban Termez, southern Uzbekistan, by local researchers and partners from Tokyo’s Rissho University.


The wall painting measures roughly 1 metre x 1 metre and features a number of people in hues of red and blue.

Mural found in Uzbekistan gives fresh glimpse of early Buddhism
The mural is believed to date from the second to third centuries [Credit: Rissho University
Uzbekistan Academic Research Group]
Images of the mural have been released with the approval of the Uzbek Academy of Sciences Fine Arts Institute, with which Rissho University collaborates.


“The mural may be part of a larger work depicting the life of Buddha,” said Haruki Yasuda, an art history professor at the university’s Faculty of Buddhist Studies. “It is a precious discovery that offers an insight into how Buddhism changed (under influences from different cultures).”

Mural found in Uzbekistan gives fresh glimpse of early Buddhism
Part of the mural unearthed at the Kara Tepe archaeological site [Credit: Rissho University
Uzbekistan Academic Research Group]
The archaeological site, situated near the border with Afghanistan, is not far from Bamiyan, where monumental Buddhist statues stood until Taliban forces dynamited them in 2001. The mural was found in a stone chamber two metres underground beside a pagoda.

Mural found in Uzbekistan gives fresh glimpse of early Buddhism
Part of the mural unearthed at the Kara Tepe archaeological site [Credit: Rissho University
Uzbekistan Academic Research Group]
Buddhism originated in India around the fifth century BC. It took 1,000 years for it to spread in a clockwise direction through Northwest Asia before reaching Japan.


Kara Tepe is located at the “crossroads of civilizations” on the Silk Road. Greek- and Roman-style human figures have been unearthed there, as well as a statue of the head of a large legendary bird in India called Garuda. Those finds also likely date from the second to third centuries. It was the first time a large mural has emerged at Kara Tepe.

Mural found in Uzbekistan gives fresh glimpse of early Buddhism
The 133-millimetre-tall head of a Garuda statue made of limestone unearthed at the Kara Tepe
archaeological site [Credit: Rissho University Uzbekistan Academic Research Group] 
Akira Miyaji, professor emeritus of Nagoya University and expert on Buddhist art in Central Asia, called the find extremely significant for studies into early Buddhist paintings. He noted that the mural combines both Eastern- and Western-style painting techniques.


“Depicting faces at an angle, along with shading and highlighting to create the impression of depth and solidity, are art techniques from Greece and Rome,” Miyaji said. “The flexible brushing and coloring style is a characteristic of art older than the Bamiyan Buddhist murals.

Mural found in Uzbekistan gives fresh glimpse of early Buddhism
The Kara Tepe archaeological site in southern Uzbekistan, where the mural was unearthed
[Credit: Rissho University Uzbekistan Academic Research Group] 
“There are also strong influences from the Hellenistic painting tradition, along with elements from India and Persia.”

Author: Yasuji Nagai | Source: The Asahi Shimbun [November 29, 2018]

Origins and spread of Eurasian fruits traced to the ancient Silk Road


Studies of ancient preserved plant remains from a medieval archaeological site in the Pamir Mountains of Uzbekistan have shown that fruits, such as apples, peaches, apricots, and melons, were cultivated in the foothills of Inner Asia. The archaeobotanical study, conducted by Robert Spengler of the Max Planck Institute for the Science of Human History, is among the first systematic analyses of medieval agricultural crops in the heart of the ancient Silk Road. Spengler identified a rich assemblage of fruit and nut crops, showing that many of the crops we are all familiar with today were cultivated along the ancient trade routes.

Origins and spread of Eurasian fruits traced to the ancient Silk Road
Excavations at the medieval site of Tashbulak are co-directed by Michael Frachetti and Farhad Maksudov;
research at the site is ongoing [Credit: Robert Spengler]
The Silk Road was the largest vector for cultural spread in the ancient world - the routes of exchange and dispersal across Eurasia connected Central Asia to the rest of the world. These exchange routes functioned more like the spokes of a wagon wheel than a long-distance road, placing Central Asia at the heart of the ancient world. However, most historical discussions of the ancient Silk Road focus on the presence of East Asian goods in the Mediterranean or vice versa.

The present study, published in PLOS ONE, looks at archaeological sites at the center of the trans-Eurasian exchange routes during the medieval period, when cultural exchange was at its highest. Additionally, scholarship has focused on a select handful of goods that moved along these trade routes, notably silk, metal, glass, and pastoral products.


However, historical sources and now archaeological data demonstrate that agricultural goods were an important commodity as well. Notably, higher value goods, such as fruits and nuts, spread along these exchange routes and likely contributed to their popularity in cuisines across Europe, Asia, and North Africa today. Ultimately this study helps demonstrate how the Silk Road shaped what foods we all eat today.

Our everyday fruits and nuts have their roots in the Silk Road

Spengler analyzed preserved ancient seeds and plant parts recovered from a medieval archaeological site in the foothills of the Pamir Mountains of eastern Uzbekistan. The site, Tashbulak, is currently under excavation by a collaborative international Uzbek/American project co-directed by Michael Frachetti, of Washington University in St. Louis, and Farhod Maksudov, of the Institute for Archaeological Research, Academy of Sciences in Tashkent, Uzbekistan.

Origins and spread of Eurasian fruits traced to the ancient Silk Road
Vendors in every Central Asian bazaar sell a diverse array of melons. These two women in the Bukhara bazaar
are selling a variety akin to the famous Hami melons of Xinjiang. There is a great deal of regional
pride associated with specific varieties of landrace melons [Credit: Robert Spengler]
The plant remains recovered from this site represent one of the first systematic studies of the crops that people were growing along the Silk Road. In the article, archaeobotanical data are contrasted with historical and other archaeological evidence in order to discuss the timing and routes of spread for the cultivated plants. These plant remains date to roughly a millennium ago and include apple, grape, and melon seeds, peach and apricot pits, and walnut and pistachio shells.

This study helps demonstrate that there was a rich and diverse economy in Central Asia during this period, including a wide array of cultivated grains, legumes, fruits, and nuts. The site of Tashbulak is located at 2100 meters above sea level, above the maximum elevations at which many of these fruit trees can be grown, suggesting that the fruit remains recovered at the site were carried from lower elevations. Historical sources attest to the importance of fresh and dried fruits and nuts as a source of commerce at market bazaars across Inner Asia.


These trade routes facilitated the spread of many of our most familiar crops across the ancient world. For example, the earliest clear archaeological evidence for peaches and apricots comes from eastern China, but they were present in the Mediterranean by the Classical period. Likewise, grapes originated somewhere in the broader Mediterranean region, but grape wine was a popular drink in the Tang Dynasty. We can now say that all of these fruit crops were prominent in Central Asia by at least a millennium ago, likely much earlier.

As Spengler points out, "The ecologically rich mountain valleys of Inner Asia fostered the spread of many cultivated plants over the past five millennia and, in doing so, shaped the ingredients in kitchens across Europe and Asia."

Central Asia is a key homeland and dispersal point for many important arboreal crops, such as apples and pistachios

Spengler also points out that many economically important fruit crops originated in the foothill forests of eastern Central Asia. For example, studies suggest that much of the genetic material for our modern apples comes from the Tien Shan wild apples of southeastern Kazakhstan, and pistachios originated in southern Central Asia.

Origins and spread of Eurasian fruits traced to the ancient Silk Road
Historical sources praise the quality of fruits from specific regions or orchards in Central Asia, such as the golden
peaches or Samarkand, the Hami melons, or the mare's nipple grapes. Many unique varieties of fruits are
 cultivated in Central Asia today, including these small Bukharan apricots, which are primarily
grown for their nutty seeds [Credit: Robert Spengler]
Despite the importance of these arboreal crops in the modern world economy, relatively limited scholarly focus has gone into the study of their origins and dispersal. The data from Tashbulak are an important contribution to that study. The article shows the importance of archaeological research in Central Asia, highlighting its role in the development of cultures across the ancient world.


In his forthcoming book, "Fruit from the Sands," Spengler traces the spread of domesticated plants across Central Asia. In the book, set to hit shelves in April 2019, he states, "The plants in our kitchens today are archaeological artifacts, and part of the narrative for several of our favorite fruits and nuts starts on the ancient Silk Road."

Excavations at Tashbulak are ongoing, with support from Washington University in St. Louis, the Max von Berchem Foundation, and the National Geographic Society. Over the next few years, the research team expects that their research will better elucidate the nature of interaction and contact in the mountains of Central Asia.

Frachetti notes, "The insights gained from this archaeobotanical study help link the juicy details of ancient cuisine to our modern tables, and in doing so highlights the long-term impact of interactions between diverse communities and regions on a global scale."

Source: Max Planck Institute for the Science of Human History [August 14, 2018]

Compared to nomadic communities, Silk Road cities were urban food deserts


Like passionate foodies who know the best places to eat in every town, Silk Road nomads may have been the gastronomic elites of the Medieval Ages, enjoying diets much more diverse than their sedentary urban counterparts, suggests a new collaborative study from Washington University in St. Louis, the Institute of Archaeology in Samarkand, Uzbekistan and Kiel University in Germany.

Compared to nomadic communities, Silk Road cities were urban food deserts
Washington University graduate student Elissa Bullion uncovers an ancient skull from a burial plot at the Medieval city
of Tashbulak in Uzbekistan [Credit: Tom Malkowicz/Washington University in St. Louis]
"Historians have long thought that urban centers along the Silk Road were cosmopolitan melting pots where culinary and cultural influences from far off places came together, but our research shows that nomadic communities were probably the real the movers and shakers of food culture," said Taylor Hermes of Kiel University, lead author of the study forthcoming in Scientific Reports and a 2007 graduate of Washington University.

Based on an isotopic analysis of human bones exhumed from ancient cemeteries across Central Asia, the study suggests that nomadic groups drew sustenance from a diverse smorgasbord of foods, whereas urban communities seemed stuck with a much more limited and perhaps monotonous menu—a diet often heavy in locally produced cereal grains.

"The 'Silk Road' has been generally understood in terms of valuable commodities that moved great distances, but the people themselves were often left out," Hermes said. "Food patterns are an excellent way to learn about the links between culture and environment, uncovering important human experiences in this great system of connectivity."

Said Cheryl Makarewicz, an archaeology professor at Kiel and Hermes' mentor: "Pastoralists are stereotypically understood as clinging to a limited diet comprised of nothing but the meat and milk of their livestock. But, this study clearly demonstrates that Silk Road pastoralists, unlike their more urbane counterparts, accessed all kinds of wild and domesticated foodstuffs that made for a unexpectedly diverse diet."

"This study provides a unique glimpse into the important ways that nomads cross cut regional settings and likely spread new foods and even cuisine along the Silk Roads, more than a thousand years ago," said study co-author Michael Frachetti, associate professor of anthropology at Washington University.

"More specifically, this study illustrates the nuanced condition of localism and globalism that defined urban centers of the time, while highlighting the capacity of more mobile communities—such as nomadic herders—to be the essential fiber that fueled social networks and vectors of cultural changes," Frachetti said.

For this study, human bones exhumed at archaeological digs in Uzbekistan and Kazakhstan were transported to Kiel University in Germany, where they were analyzed by Hermes. To be thorough, he also collected previously published isotopic data for the time period to bring together a complete regional picture.

"Prior to this study, there were massive gaps in what we knew about human dietary diversity along the Silk Roads," Hermes said. "The datasets were simply not there. We were able to greatly increase the geographical coverage, especially by adding samples from Uzbekistan, where many of the important routes and population centers were located."

The study draws upon field work and museum collections as part of a longstanding scientific partnership between Washington University and the Institute of Archaeology in Samarkand, Uzbekistan.

The study's assessment of individual dietary regimens is made possible by studying the isotopic signatures in ancient human bones, allowing the researchers to unlock a trove of information about the food sources, including the proportions and types of plants and animals consumed by individuals over the last decades of life.

Stable isotope analysis is the "gold standard" for tracing ancient diets. Makarewicz, a specialist in the technique, has applied it to understanding major evolutionary transitions from hunting and gathering to agriculture in the Near East. She is starting a new interdisciplinary ERC research project exploring the spread of herding across Eurasia.

Other co-authors include Elissa Bullion, a doctoral student in anthropology at Washington University and two researchers from the Uzbek partnership: Farhod Maksudov and Samariddin Mustafokulov.

Hermes, who has worked with Frachetti on archaeological digs across Central Asia for more than a decade, used these isotopic analysis techniques on human bones recovered from about a dozen nomadic and urban burial sites dating from the 2nd to 13th centuries A.D.

The burial sites were associated with a wide range of communities, climates and geographic locations, including a recently discovered settlement high in the mountains of Uzbekistan, the Otrar Oasis in Kazakhstan and an urban complex on the lowland plains of Turkmenistan.

While previous archaeological excavations at these sites have confirmed the ancient presence of domesticated crop plants and herd animals, their importance in urban diets was unknown. Isotopic analysis, however, shows how important these foods were over the long-term.

"The advantage of studying human bones is that these tissues reflect multi-year dietary habits of an individual," Hermes said. "By measuring carbon isotope ratios, we can estimate the percentage of someone's diet that came from specific categories of plants, such as wheat and barley or millet. Millets have a very distinctive carbon isotope signature, and differing ratios of nitrogen isotopes tell us about whether someone ate a mostly plant-based diet or consumed foods from higher up on the food chain, such as meat and milk from sheep or goats."

This study discovered interesting dietary differences between urban settlements along the Silk Road, but surprisingly little dietary diversity among individuals living within these communities. Perhaps driven by the limits of local environments, food production networks or cultural mandates, most people within each urban setting had similar diets.

Diets of individual nomads within the same community were found to be much more diverse. These differences, perhaps a function of variable lifetime mobility patterns, the availability of wild or domesticated food options or personal preferences, suggest that nomadic groups were not as bound by cultural limitations that may have been imposed on urban dwellers, Hermes said.

"Nomads and urbanites had different dietary niches, and this reflects a combination of environment and cultural choices that influenced diet across the Silk Roads," Hermes said. "While many historians may have assumed that interactions along the Silk Road would have led to the homogenization of culinary practices, our study shows that this was not the case, especially for urban dwellers."

For now, Hermes, Frachetti, Makarewicz and their collaborators in Samarkand look forward to applying these isotopic techniques to new archaeological mysteries across Central Asia.

"We hope our results lead to a paradigm shift in how historical phenomena can be examined through the very people who made these cultural systems possible," Hermes said. "The results here are exciting, and while not the final word by any means, pave a new way forward in applying scientific methods to the ancient world."

"For close to 10 years our academic collaboration has yielded fascinating new discoveries in archaeology and has also fostered new international partnerships, such as the one spearheaded by Taylor Hermes, to carry out archaeological science at Kiel," Frachetti said. "This international approach is what enables us all—as a team—to maximize the scientific potential of our collaborative fieldwork and laboratory studies in Uzbekistan for the advancement of historical and environmental knowledge more globally."

Source: Washington University in St. Louis [April 18, 2018]